For bioremediation of H2S toxicity, the bacterium that belongs to Chromatiaceae and Chlorobiaceae can be mass cultured and can be applied as pond probiotic. Being autotrophic and photosynthetic, mass culture is less expensive and the cultured organisms can be adsorbed on to the sand grains and applied so that they may reach the pond bottom to enrich the hypolimnion and ameliorate H2S toxicity (Singh and Radhika 2001).