An Anaerobic Sequencing Batch Reactor (ASBR) with a 1-L active volume was set up and operated at 35 °C under dark conditions to prevent photo degradation. The reactor was operated with a 24-hour cycle consisting of fill (10 min), react (23 h), settle (45 min) and decant (5 min), and it was mixed continuously by a magnetic stirrer at 90 rpm.