MS
media were prepared in two different sucrose
concentrations viz. 3% and 6% respectively.
Kinetine (KIN) was used as a induced hormone for
microtuber production. MS media were supple-
mented with 1, 2, 3, 4 mg/L of Kinetine (KIN) for
the present study. For each concentration of
hormone and sucrose 20 replicates were prepared.
Tuberization media were poured into well establish
plantlet. Fifty percent of the cultures were incub-
ated under 16 hour of photoperiod at 2500-3000 lux
and rests of the materials were incubated under
dark condition at 17± 3
0
C. Microtubers were
harvested after 45-55 days of incubation. Data were
recorded on days to shoot formation, number of
shoot per explant, shoot length, days to micro tuber
formation, number of micro tuber per explant and
average weight of microtuber.
MS media were prepared in two different sucrose concentrations viz. 3% and 6% respectively. Kinetine (KIN) was used as a induced hormone for microtuber production. MS media were supple- mented with 1, 2, 3, 4 mg/L of Kinetine (KIN) for the present study. For each concentration of hormone and sucrose 20 replicates were prepared. Tuberization media were poured into well establish plantlet. Fifty percent of the cultures were incub- ated under 16 hour of photoperiod at 2500-3000 lux and rests of the materials were incubated under dark condition at 17± 30C. Microtubers were harvested after 45-55 days of incubation. Data were recorded on days to shoot formation, number of shoot per explant, shoot length, days to micro tuber formation, number of micro tuber per explant and average weight of microtuber.
การแปล กรุณารอสักครู่..
