In this study, we designed two prim

In this study, we designed two primers sets and three different sets of probes, based on previous analyses [3,13–16], to detect the etiologic agents of tinea unguium by real-time PCR. Our primers and probes targeted the internal transcribed spacer 1 (ITS1) region in order to detect a wide range of pathogenic fungi and the two major causative dermatophytes, T. rubrum and T. mentagrophytes human-type. We compared the results of our new PCR analyses with the findings obtained by microscopic examination and culture of 42 clinical specimens.