2.6. In Vivo Toxicity Study. In vivo toxicity study was carried
out using Balb/C mice (20 ± 3 g, n = 4). Animals were
injected intraperitoneally with the test compounds affording
doses of 50mg/kg of body weight with one group being
given DMSO (DMSO control) in the same concentration
along with serum-free medium, while the other group of
control was continued without any solvent treatment. Mice
were observed for 96 h to record postinjection discomfort,
if any. At the end of 4th day, the gross body weight was
recorded. Animals were bled through heart puncture, and
the blood, and serum were subjected for hematological and
physiological analyses.