Fig. 2. Effects of CYP2A6 and CYP2A13 on the NNK-induced supF gene mutation. (A) The
supF mutagenesis assay was carried out as schematically described. (B) Following the
procedure, A549 and H1437 cells were left uninfected (no virus) or infected with one of
the recombinant adenoviruses (Ad-Mock, Ad-CYP2A6, and Ad-CYP2A13). After
transfection with pZ189, the cells were treated with NNK (200 μM) for 24 h. Finally,
pZ189 was rescued from cells for the supF mutagenesis assay (see Supplementary
material in detail). The experiment was repeated three times. SupF mutation rates are
presented as means±SD. *pb0.05, **pb0.01, ***pb0.001.