and adults of gastrointestinal nema

and adults of gastrointestinal nematodes.
One of the advantages of in vitro tests of A. sisalana extract is to assess its effect on L1 stage.
The presence of bioactive compounds in the leaves of A. sisalana makes this plant an interesting research target for the use of its extract for the development of products with high aggregate value, such as natural antiparasitic agents.
In this respect, the highest concentrations produced inhibition levels above99%in the EHT,LDT and LFIT, demonstrating its potential for use to control nematodes, in
conformity with the recommendations of the World Association for the Advancement of Veterinary Parasitology(WAAVP), according to which this plant has high antiparasitic efficacy.
Due to the reports of biological activity of extracts rich in saponins on various organisms as well as the significant results in vitro and in vivo in various studies of our research group, we believe that A. sisalana should be further investigated. According to the previous experiments, the risk of toxicity is quite unlikely at low doses, but pre-clinical and clinical evaluations need to be performed.
The active substances need to be confirmed as well the mechanism of action on nematodes.
The anthelmintic efficacy could be evaluated in vivo in an experiment of longer duration in the field. Thus, the action of A. sisalana in stools could be verified.