The enzymatic coproduction of biodiesel and glycerol carbonate by transesterification of soybean oil and
dimethyl carbonate (DMC) has been studied in a solvent-free system. The effects on biodiesel and glycerol
carbonate conversion of reaction conditions including the kind of enzyme, the amount of enzyme, the
molar ratio of DMC to soybean oil, the reaction temperature, and water addition were investigated. The
optimal conditions for biodiesel and glycerol carbonate were 20% Novozym 435, 10:1 molar ratio of DMC
to soybean oil, and 0.7% water addition. Under these conditions, the conversions of 96.4% biodiesel and
92.1% glycerol carbonate have been achieved after 48 h.