the method developed by Chomczynski

the method developed by Chomczynski (1993). Messenger RNA
was purified using the Dynabeads mRNA purification kit (Invitrogen)
and the quality of total and mRNA was verified using a
2100 Bioanalyzer (Agilent technologies). The construction of the
whole transcriptome libraries, the sample preparation for RNASeq
sequencing and the sequencing were performed according to
the Ion Torrent protocols using the Ion Torrent Personal Genome
Machine (PGM).