The rats were randomly divided into

The rats were randomly divided into four groups of six rats each. Group I:
Normal untreated rats. Group II: Rats were orally treated with p-coumaric acid
(8 mg/kg body weight) dissolved in 2 ml of 0.5% dimethyl sulfoxide (DMSO) solvent
using an intragastric tube daily for a period of 7 days. Group III: Rats were injected
subcutaneously with ISO (100 mg/kg body weight) dissolved in 2 ml of saline at an
interval of 24 h for 2 days (on 8th and 9th day). Group IV: Rats were orally pretreated
with p-coumaric acid (8 mg/kg body weigth) dissolved in 2 ml of 0.5%
DMSO using an intragastric tube and then injected subcutaneously with ISO
(100 mg/kg body weight) at an interval of 24 h for 2 days (on 8th and 9th day).
Normal control and ISO control rats were administered 2 ml of 0.5% DMSO alone
orally using an intragastric tube daily for a period of 7 days of the experiment.
The dosage (8 mg/kg body weight) and duration of pretreatment of p-coumaric acid
(7 days) was based on our previous study (Roy and Stanely Mainzen Prince, 2013).
At the end of the experimental period, after 12 h of second ISO injection,
electrocardiogram (ECG) was recorded in all groups of rats after injecting
anesthetic, ketamine hydrochloride (100 mg/kg body weight). Then, all the rats
were sacrificed by cervical decapitation. Blood was collected in a tube without
anticoagulant for the separation of serum. Heart and liver tissues were excised
immediately and rinsed in ice-chilled saline and stored at 80 C for further
biochemical estimations.
2.5. Estimation of serum cardiac sensitive marker
The level of cTnT in serum was estimated using a kit by electro chemilumines