In our previous study, we predicted protein–protein interactions of L. interrogans strain Lai chemotaxis proteins by four computational methods that calculate the functional linkages. This prediction of the interaction network of L. interrogans strain Lai chemotaxis and motility associated proteins demonstrated that the 11 MCPs are likely to link to each other and that CheB2 links solely to CheR1 and CheB3, suggesting that CheB2 may not function alone but under certain physiological conditions may require CheB3 and CheR1 for function [