Shoot proliferation from seedling e

Shoot proliferation from seedling explants of Dionaea muscipula Ellis (Venus Fly Trap) was induced and maintained on Linsmaier and Skoog medium with 10 μM kinetin and 0.5 μM NAA. Proliferation was influenced by cytokinin type and concentration, with kinetin being more effective than BAP. The pH of the media had a dramatic effect, with 5.7 being optimal, while varying light level had only a moderate effect. Omission of nitrogen from the medium reduced proliferation, but this was partially restored by the addition of casein hydrolysate. Reducing salt concentration to half or one-tenth also reduced proliferation. At the lowest salt level, the addition of casein hydrolysate again partially restored proliferation. Plantlets could be established in potting media and successfully grown under glasshouse conditions.