CONCLUSIONS
Microbially synthesized silver nanoparticles were about 10-20 nm in size which showed highest antimicrobial activity against Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae, Proteus vulgaris and Escherichia coli. The “biogenic” approach is further supported by the fact that the majority of the bacteria inhabit ambient conditions of varying temperature, pH, and pressure. The particles generated by these processes have higher catalytic reactivity, greater specific surface area, improved contact between the enzyme and metal salt in question due to the bacterial carrier matrix and smaller size of silver nanoparticles. The smaller silver nanoparticles showed stronger antibacterial activity compared to the chemically synthesized silver nanoparticles (60-80 nm). The bacterium was highly resistant to silver cations. These silver nanoparticles were of high purity, making them potentially useful for biological applications. The application of silver in combination with microbial system would be effective in enhancing its anti-microbial activity.