3.2. Sensitivity testing of mutiple

3.2. Sensitivity testing of mutiplex PCR and monoplex PCR
For the 10-fold dilution series, the highest dilutions at which mutiplex PCR showed positive results were 10−2, 10−3, 10−4, and 10−3 for Aichi virus, HPeV, EVs, and HBoV, respectively. On the other hand, the positive results of highest dilution in monoplex PCR were 10−3 for Aichi virus, 10−4 for HPeV, 10−4 for EVs, and 10−3 for HBoV (Fig. 2). Taken together, the results indicated that the sensitivity of multiplex PCR decreased slightly for Aichi virus and HPeV compared to monoplex PCR.