Electrospun nanofibers are promising nano-biocomposite material for immobiliza- tion of microorganism since they can afford large surface area-to-volume ratios, pore, and surface functionalization and several active sites for contact or attach- ment. It was inferred from the above reports that increase in initial cell viability numbers increases the removal performances. Due to the lack of internal and external mass transfer resistance, better biodegradation percentage was obtained by immobilized microbial cells. Therefore, biohybrid materials are potentially an attractive, reusable, modest, highly robust, and suitable for water and wastewater treatment with storable and improvable properties. In this chapter, finally, we conclude that the incessant research and enhancement in nanofiber technology will become an effective and practical technology for wastewater treatment of con- taminant removal in bioremediation process.