Although the GFP has proven to be a useful tool on its own, combining the use of GFP technology with other luminescent markers or probes is also promising to provide answers to challenging questions in environmental research studied community structures with GFP and tetra- methylrhodamine-labelled probe, GAM42a and simultaneously detected GFP-marked P. putida Green3l and cells belonging to beta and gamma subclasses of Proteobacteria.