The present study though confirmed the presence of
invertase enzyme activity in fresh Pressmud and spent
yeast, residual specific invertase activity from spent yeast
when cultured on fresh pressmud was found to be higher
in titers. Solid state fermentation triggered on press mud
as substrate gave maximum specific activity at 72 h of
spent yeast cultivation. Combination of spent yeast &
fresh cultured yeast (7:3) as inoculum gave maximum invertase
activity at 72 h. Till 50% w/w of spent yeast on
pressmud favored maximum invertase production. The
crude enzyme activity was optimized in terms of temperature
and pH. Impact studies for the metal ions
showed fair increase in the residual enzyme activity in
presence of metal ions and marginal increase by detergents.
About 10 folds of purification of crude enzyme activity
obtained by means of three phase partition method