We used four nuclear loci (both rRNA and protein
coding genes) that have previously proved useful for the molecular investigation of taxa belonging
to the genus Penicillium at various evolutionary levels. The internal transcribed spacer region
(ITS1–5.8S–ITS2), domains D1 and D2 of the 28S rDNA, a region of the tubulin beta chain gene
(benA) and part of the calmodulin gene (cmd) were amplified by PCR and sequenced