Effects of dietary supplementation of probiotic Shewanella
colwelliana WA64, Shewanella olleyana WA65 on the innate
immunity and disease resistance of abalone, Haliotis discus
hannai Ino
abstract
The effects of dietary administration of two probiotics, Shewanella colwelliana WA64 and Shewanella
olleyana WA65, on the innate immunity of abalone (Haliotis discus hannai Ino), and survival of juvenile
abalone challenged with Vibrio harveyi have been studied. Two groups of abalone were fed with three
different diets: one control, and two diets supplemented with 109 cell g1 of probioticWA64 (WA64 diet)
and WA65 (WA65 diet) for up to four weeks. Results showed that abalone fed diets containing
S. colwelliana WA64 and S. olleyana WA65 had led to an enhanced cellular and humoral immune
response, notably higher haemocytes, respiratory burst activity, serum lysozyme activity and total protein
levels were recorded after one week of probiotic administration. On the other hand, mortality after
the challenges with V. harveyi in the group fed with control diet ranged from 77 to 80%, while mortality
rates observed in the groups fed with diets supplemented with WA64 and WA65 ranged from 27 to 50%
and 30e43%, respectively. The results demonstrated potential for S. colwelliana WA64 and S. olleyana
WA65 to improve innate immunity and disease resistance in H. discus hannai.