Forty-one isolates of Bacillus species were isolated from raw milk,
analyzed using the spot on lawn and agar diffusion method in terms of their
general inhibition effects to test bacteria (Escherichia coli TISTR 887 and
Staphylococcus aureus TISTR 517). The results demonstrated that most
isolates are effective against Gram-positive and Gram-negative bacteria
whereas their extensive inhibition effect is particularly against Grampositive
bacteria. Only 2 effective thermotolerant isolates, BA8 and BA16,
exerted broad spectrum antibacterial activities against both test bacteria.
Based on biochemical and physiological properties, they were classified as
Brevibacillus laterosporus and Geobacillus thermoglucosidasius,
respectively.