Among them, the best result for eth

Among them, the best result for ethanol production was
obtained when 15 g DS of sugarcane shoots and leaves was pretreated by using two step of 2% w/v NaOH solution autoclaving
followed by 2% w/v H2SO4 solution autoclaving. The highest ethanol concentration 30.40 g/L (92.65% in fermentation efficiency) was
obtained from reducing sugar 89.25 g/L at 48 h. Moreover, the washing step of solid residue after pretreatment could reduce furfural
and hydroxymethylfurfural (HMF) in all pretreatment methods when compared to unwashing solid residue after pretreatment.
Key words: Sugarcane shoots and leaves, lignocellulosic biomass, pretreatment and enzymatic saccharification, cellulosic ethanol
production.