Analyses of total coliform and E.co

Analyses of total coliform and E.coli in these mahi-mahi samples were conducted using the three-tube most probable number (MPN) methods. LST and BGLB incubated at 35c for 48 h were used for presumptive and confirmation tests for total coliform, respectively. E.coli was determined by using the LST and EC incubated at 35c and 44.5c for 48 h, respectively. Cultures that showed positive production of gas in BGLB or EC were then confirmed by eosine methylene blue agar and IMViC test.