Separation was performed using the same gradient described above; here, an Ultimate XB-C18 column (3.5 μm, 2.1 mm × 150 mm, Welch) and a Phenomenex precolumn (2 mm × 4 mm, Torrance, CA) were used at 30 °C.
Separation was performed using the same gradient described above; here, an Ultimate XB-C18 column (3.5 μm,2.1 mm × 150 mm, Welch) and a Phenomenex precolumn (2 mm × 4 mm, Torrance, CA) were used at 30 °C.