Fig. 3. SIRT1 expression in HEK239

Fig. 3.
SIRT1 expression in HEK239 cells exposed to compounds 1, 3, 4, 8 and 10 at a concentration of 20 μM for 24 h. C is representative of the control group. SIRT1 protein expression was evaluated using anti-SIRT1 antibodies as described in the Experimental section. One representative western blot from three independent experiments is shown, with β-actin serving as an internal control. Fold induction was calculated by Sirt1/β-actin (% control). Data are presented as the mean ± S.D. from triplicate independent experiments. The sign * means p < 0.05 versus the control group.