This technique is very sensitive, effective. It provides great selectivity against matrix interference5.This technique has the properties like reproducibility, repeatability and specificity which are very advantageous. This technique improves the limit of detection and limit of quantification values6.This technique is rapid and sensitive. It requires less sample preparation by which different types of metabolites are accurately measured. It is having high through output7.By this technique the classical mass spectra can be obtained. In this low electric field were observed. Q2 acts as a good focusing device, it is useful for mixture screening. This technique is tolerated to high pressure ionization sources8.MS/MS allows improvement in signal to noise ratio(S/N) for detecting compounds which are present in mixtures. This technique improves the detection limit for complex mixtures9.