Anthocyanins were identified and qu

Anthocyanins were identified and quantified by Varian LC system (USA) equipped with a ProStar 230 solvent delivery module, and ProStar 330 PDA Detector. Anthocyanin separationwas done in an OmniSpher C18 column (250 _ 4.6 mm inner diameter, 5 mm,Varian, USA) protected with guard column (ChromSep 1 cm_ 3 mm, Varian, USA) using 0.5% water solution of phosphoric acid as a solvent A and 100% HPLC grade methanol as a solvent B (elution conditions: 0e38 min from 3 to 65% B; 38e45 min, 65% B; flow rate 1 mL min_1, injection volumes were 20 mL)