Materials and MethodsStandards and

Materials and Methods
Standards and reagents
Ethanol (60%, v/v) was purchased from Fisher Scientific (Leicestershire, UK), Deionized distilled water (ddH2O), distilled water was prepared with Favorit W4L water distillation system from Genristo (Nottingham, UK). 1,1-Diphenyl-2-picrylhydrazyl (DPPH), sodium acetate (C2H3NaO2•3H2O), acetic acid, 2, 4, 6-tripyridyl-s-triazine (TPTZ), hydrochloric acid (HCl), ferric chloride (FeCl3•6H2O), ferrous sulfate (FeSO4•7H2O), aluminium trichloride (AlCl3), sodium nitrite (NaNO2) were purchased from Sigma Chemical Co. (St. Loius, MO, USA). Folin-Ciocalteu reagent, sodium carbonate, gallic acid, ascorbic acid, sodium hydroxide (NaOH) and quercetin were purchased from Sigma-Aldrich (Steinheim, Germany).