The expression level of P53 showed a decline pattern among the treated groups relative to the control group (Table 2). The two way ANOVA analysis based on F test showed a P value of 0.284 between groups, which is not significant. There were observed differences between groups receiving different concentrations of SP; however, it was not sufficient enough
to be significant. LSD between time showed a significant difference between first month and third month with a P value equal 0.025 (< 0.05) (Table 3).