Effect of HC on infected cells. The

Effect of HC on infected cells. The effect of HC on IBV Beaudetteinfected
cells was evaluated. The cells were infected with IBV at a
multiplicity of infection of 2 at 378C for 1 h, after three washes with
PBS. The infected cells were treated with serially diluted HC at 378C at
1 h post infection. The infectivity of the viruses was calculated with
plaque assays, after the cells were washed with PBS. In parallel, GD
(Zhengda Pharmacy, Shanxi, China) and LiCl (Sigma, CA, USA) at
different concentrations were used as controls for the above-mentioned
infection analysis.