In the pulp, transcript levels of M

In the pulp, transcript levels of MaERF3, -7, -9, and -14 were higher in ethylene-treated fruit, and their transcript levels in natural, ethylene-induced, 1-MCP-delayed ripening, or combined 1-MCP and ethylene-treated fruit clearly increased with ethylene evolution, with a more obvious accumulation for MaERF9 (Fig. 3 and Supplementary Fig. S6). MaERF2, -6, -10, -12, and -13 transcript levels slightly increased in ethylene-treated fruit, and their transcript levels in natural, 1-MCP-delayed ripening, or combined 1-MCP and ethylene-treated fruit decreased or changed only slightly during ripening (Fig. 3 and Supplementary Fig. S6). MaERF1, -4, -5, -8, -11, and -15 transcript levels decreased after ethylene treatment, following the increase in ethylene production, with more remarkable decrease for MaERF1, -11, and -15 (Fig. 3 and Supplementary Fig. S6). Based on these 15 MaERFs expression patterns during fruit ripening, two most ripening-related banana ERF genes, including ripening-induced MaERF9 and ripening-downregulated MaERF11, were selected and focused upon for the following analysis.