After the heat treatment was finished, samples were immediatelly moved and cooled at room temperature, then
plated with growth medium. Samples were homogenized by vortex mixer for 2 minutes, and 1 ml of them was diluted
into 9 ml 8.5% sterile NaCl solution and homogenized to obtain sample with 10-1 dilution factor. Plating was done
for 10-1 until 10-6 dilution. One ml of samples from different dilutions was moved into sterile petridish and poured with
sterile NA solution to grow bacteria population, while for growing mould and yeast, sterile PDA medium solution was used.