Anthocyanin components in transgeni

Anthocyanin components in transgenic gentian flowers
To investigate anthocyanin components in the transgenic gentian, the hydrolysed flower extracts were subjected to HPLC analysis. The transformation host, gentian cv. ‘Albireo’, accumulated major delphinidin derivatives and minor cyanidin derivatives in its petals (Fig. 4A). No significant change of total anthocyanin accumulation was detected in the flowers of clone no. 1. On the other hand, the flowers of clone no. 15 had reduced total anthocyanin accumulation (ca. 30% of the wild type), indicating that the reduction of delphinidin derivatives by suppression of F3′5′H gene did not lead to an increase in cyanidin derivatives ( Fig. 4A). The profiles of flavones, accumulating abundantly in gentian petals, were not affected by the downregulations of F3′5′H and/or 5/3′AT genes ( Fig. 4B).