Abundance of leaf endophytic fungi was depicted by frequency of isolation, in which calculated by the percentage of samples with certain endophyte. The frequency of isolation then compared between aphid free plants and plant with aphids. The collected endophytic fungi were stored on test tube containing PDA and store at 5?C. Propagation was carried out by reculturing this isolate on PDA. The 14-days old colony of endophyte was used for inoculation.