The obtained nucleotide sequences were analyzed by
comparison with the nucleotide sequence in the Gen-
Bank database using BLASTN (http://blast.ncbi.nlm.nih.
gov/blast/Blast.cgi), and all the nucleotide sequences
from this study were submitted to the GenBank database.
The nucleotide sequences of each region were aligned,
and the percentage of the intra-specific variation was calculated
using BioEdit Sequence Alignment Editor, Version
7.1.9. A phylogenetic tree was constructed via the
neighbor-joining method using Kimura’s 2 -parameter
model implemented in MEGA6.06, and the tree was
tested using 1000 bootstrap replicates and compared with
the reference sequence (clade A, B and C). Pediculus
schaeffi accession no. AY695999 was an outgroup.