L. acidophilus present in fermented food mixtures were enumerated using DeMan–Rogosa–Sharpe (MRS) medium. One gram of fermented slurry was added to 9 ml sterile normal saline solution. Further dilutions up to 10−10 were made. Each dilution (1 ml) was pour plated in sterilized petriplates, incubated at 37 °C for 24 h and the colonies were counted by pour plating method using a colony counter.