In order to simulate a natural chal

In order to simulate a natural challenge
infection, green peach aphids (Myzus persicae) were raised on healthy turnip
(Brassica rapa) and transferred onto
healthy tobacco for adaptation. Aphids
were starved and transferred to tobacco
plants infected with CMV-16 as described
previously (14). Aphid-infested, CMV-16-infected tobacco plants were transplanted
into the field and were randomly distributed among the test plants to allow aphid
spread and “natural” challenge infection. A
total of 15 tobacco plants were used in
each test plot.