concentrations (0.12–1 mg/mL), the inhibition was 100%, with a significant
difference (p < 0.05) between these and the lowest concentrations
(0.015–0.06 mg/mL),
which were not effective and did not mutually differ statistically.
The average feeding inhibition percentage in the negative control, with distilled water, was 26.8% (Table 3).
The LC50 and LC95 in the LFIT were 0.053 (0.037–0.078) and 0.24 (0.138–0.752) mg/mL, respectively (Fig. 4).
The motility inhibition of the H. contortus adults treated with the A. sisalana extract concentrations varied from 0% to 100% (Table 4).
There was an influence of the exposure time on movement reduction of the nematodes except in the first 24 h after treatment at a concentration of 75 mg/mL, in which all the parasites were killed,
and at a concentration of 9.375 mg/mL, which did not show the
same response pattern as the other concentrations.
However, the inhibition at this concentration after 48 h did not differ significantly
from the treatment with ivermectin (positive control).
After 6 h of exposure to the A. sisalana extract, the motility inhibition percentages
were 0% from 4.687 to 37.58 mg/mL and 16.7% at 75 mg/mL,
with no significant difference in the inhibition percentage (0% and 16.67%) among the concentrations and between them and ivermectin.
However, after 24 h, the inhibition percentages of the A. sisalana extract were higher (p < 0.05) than for ivermectin at concentrations from 18.75 to 75 mg/mL. The inhibition percentages of the distilled water (negative control) were 0%, 33.3% and 75.0% for 6, 24 and 48 h, respectively. After 24 h there is no significant difference
among negative control, ivermectin and A. sisalana extract at 4.687 mg/mL (Table 4). The average motility inhibition of 66.7% of ivermectin on H. contortus after 48 h of treatment indicates the possibility of anthelmintic resistance in this nematode population