Analysis of ESR data
Earlier methods of ESR analysis were used as described (18). Intensities were measured as the distance between top and bottom points of the first derivative for monitoring variations in the peak height of ESR signals as a function of the magnetic field. Quantitative assessments of free-radical concentrations were made according to the following equation,
Nd=K[Ho(ΔH2) A/2]/[Hm×Ge √ PH ]
where Nd = number of radicals, K = 103/cm, Ho = peak magnetic field in gauss, ΔH = peak-to-peak width, Hm = modulation field, PH = 1.008mW, Ge = detector gain = 3.17×105, concentration = unpaired electrons/lyophilized blood (g) or spin/lyophilized blood (g), and A = peak height of signals/weight.