The objective of this work was to make a contribution to study the potential of the sequential injectionlab-
on-valve (SI-LOV) format for the miniaturization of enzymatic assays, by using different measurement
modes (peak height and initialrate-based measurement). A LOV system was developed for the enzymatic
assay of ethanol in beverages, based on the conversion of ethanol to acetaldehyde by alcohol
dehydrogenase, using spectrophotometric detection. The use of the kinetic-based approach permits the
applicability of the enzymatic determination to samples with intrinsic absorption, with a higher determination
throughput.