PCR was conducted as follows: 2 min

PCR was conducted as follows: 2 min at 94C; 25
cycles of 20 s at 94C, and 1 min at 72C. a Effect of pH on
PCR amplification with Tce DNA polymerase. Amplification
of the a 2 kb k DNA fragment was performed in 50 ll
containing 50 mM Tris/HCl, 5 mM MgCl2, and 0.01% Triton
X-100, at the indicated pH values. b Effect of KCl on PCR
amplification with Tce DNA polymerase. Amplification of a
2 kb k DNA fragment was performed in 50 ll containing
50 mM Tris/HCl (pH 7.8), 5 mM MgCl2, and 0.01% Triton
X-100 with the indicated KCl concentrations. c Effect of