Turbidity The experiments were carr

Turbidity
The experiments were carried out in a 50-ml bottle. Temperature was maintained at 20°C and a turbidity sen- sor positioned through the bottle wall, to avoid distur- bances during stirring. Turbidity changes accompanying the nano-emulsion formulation were observed using a turbidity meter (Analite NEP 160, McVan Instruments, Mulgrave, Australia). This apparatus used light in the near infrared region (860 nm) so that any particle in sus- pension in the fluid reflected the incident beam at 180° back to a sensitive electronic receptor. A measurement system ensured the continuous monitoring of turbidity (Almemo 8990-8 V5, Ahlborn, Holskirchen, Germany). This data logger was coupled with a PC equipped with an AMR WinControl software (adapted for Almemo). Data were collected automatically every second during 1,000 seconds, then every five seconds. All runs were carried out at least in triplicate.