Previous publications (Eldridge et

Previous publications (Eldridge et al., 2002; Cremin
and Zeng, 2002) have documented our high-throughput
purification methods applied to the production and
analysis of the natural products libraries from plants.
We have been using these methods to increase the rate
of discovery of novel, biologically active compounds in
various drug discovery collaborations. During the bioassay-
guided fractionation of biologically active fractions,
micrograms are used to locate activity, but one or more
milligrams of a compound are needed for structure elucidation
when using 3 or 5 mm conventional NMR
probes. Therefore, increasing the NMR sensitivity is a
crucial factor for the miniaturization of the structure
elucidation and dereplication of the mass-limited samples.