2.6. Phage therapy assays
Phage therapy was performed using one phage alone (VP-1, VP-2 or
VP-3) and with phage cocktails (two or all the three phages mixed together,
each phage at the same concentration) using the bacterium
V. parahaemolyticus as host, at aMOI of 100. The assayswith two phages
were performed with the following phage combinations: VP-1/VP-2,
VP-1/VP-3, VP-2/VP-3 and VP-1/VP-2/VP-3 phages. In order to obtain
a MOI of 100, 20 μL of the overnight V. parahaemolyticus culture
(105 CFU mL−1) and 300 μL of the phage suspension (107 PFU mL−1)
were inoculated into sterilized glass Erlenmeyers with 30 mL of TSB
medium and incubated at 25 °C without agitation in the dark (test