(to increase the coconut oil yield), combination of treatments (enzyme
treatment followed by chilling at 5 C and thawing to ambient
temperature) was employed. It was observed that coconut milk
treated with 0.1% of protease at 25 C followed by chilling and
thawing yielded 91%. Whereas, the highest yield of 94.5% was observed
in the coconut milk sample treated with 0.1% of protease at
37 C followed by chilling and thawing to ambient conditions.
Optimum activity of enzyme was observed at 37 C hence, complete
destabilization of milk emulsion is occurred. Chilling and
thawing is also important for complete destabilization of the enzyme
treated emulsion, as packing of globules during chilling is
necessary to facilitate oil separation (Gunetileke and Laurentius,
1974). Thus it may be noted that combination of treatments (enzyme
followed by chilling and thawing) was more effective for
the destabilization of coconut milk emulsion compared to all other
treatments.