Extraction of total muscle proteins was performed in a
denaturation buffer (8.3M urea, 2M thiourea, 1% DTT, 2%
CHAPS) as described by Bouley et al. (2004). Muscle protein
concentration was determined by spectrophotometry with
the Bradford assay (Bradford, 1976). Total extracted proteins
were stored at2208C before analysis. A monoclonal primary