The process of CXF, including the introduction of xenon gas under a pressure of 1.0 MPa at 1 C for 1 h frozen to 20 C, was studied. Typical restricted diffusion phenomenon were determined using a nuclear magnetic resonance method, and it was found that CXF barley coleoptile cells were similar to those of fresh barley. In contrast, the FAP barley coleoptile cells showed unrestricted diffusion phenomenon. The results from X-ray radiographic images also suggested that the CXF process can preserve the shape of barley coleoptile tissues and, additionally, it was found that xenon hydrate formation occurred inside the cells and intercellular spacing of such cells. It can therefore be suggested that the assumed process of xenon hydrate formation in plant cells results in CXF being more effective for the preservation of plant cells and their tissues than the FAP process.