4. Conclusion
The biopretreatment with the ligninolytic enzymes in vitro in this study could avoid the loss of hemicelluloses and cellulose, which will enhance the sugar yield after hydrolysis in theory. Our results indicated that ligninolytic enzyme could inhibit the acitivity of cellulase and the hydrolysis of corn stover. Removing the lignin-degrading enzyme from the reaction system by washing after biopretreatment could eliminate the inhibition. Analysis of FT-IR and XRD revealed the mechanisms how the biological pretreatments improved the sugar yield from corn stover hydrolysis by cellulase. This study provided a promising method for lignocelluloses bio-pretreatment and enzymatic hydrolysis.