Then the agar plates were allowed t

Then the agar plates were allowed to solidify at 4 C for 1 h. Five equidistant holes were made in the agar using sterile cork borers (Ø ¼4 mm). The extracts (50 mL) were prepared at 1, 2.5, 5 and 10 g/100 mL concentrations in absolute methanol and were applied to the holes using a pipettor and absolute methanol without herb extract was used as a control.
Again, 50 mL of the essential oil at 0.2, 0.5, 1 and 2 g/100 mL concentrations in dimethylsulfoxide (DMSO, Merck, Germany) were filled to the holes using a pipettor and DMSO without the essential oil was used as a control.
Y. enterocolitica, C. albicans and S. cerevisiae was incubated at 25 C for 14–24 h. The other microorganisms were incubated at 35 C for 18–24 h.
Inhibition zones which formed on the medium were measured in millimeters (mm).
All determinations were carried out in duplicate and the results were averaged.