Reducing Power testThe reducing pow

Reducing Power testThe reducing power of the extracts was determined accordingto the method previously described by Oyaizu (1986). The assayis based on the reduction of Fe3+by antioxidant compounds. Var-ious concentrations of the extracts (0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7,0.8, 0.9 and 1 mg/mL) were mixed with phosphate buffer (1 ml,0.2 M, pH 6.6) and 1 mL of 1% potassium ferricyanide. The mix-ture was incubated for 20 min at 50◦C. After incubation, 1 mL of10% trichloroacetic acid solution was added to the mixture. Themixture was further centrifuged at 3000 min−1for 10 min. Theobtained supernatant (2 mL) was mixed with double distilled water(2 mL) and 0.1% FeCl3solution (0.4 mL). Absorbance was measuredat 700 nm. The blank was prepared by using water instead of anextract.