The three classical pheromone-driven behaviors in
Drosophila are aggregation, male courtship, and female
post-mating behaviors (Table 1). Aggregation behavior is
observed in both sexes and is induced by an attractive
volatile pheromone, cis-vaccenyl acetate (cVA). This
pheromone is produced in the reproductive organs of
the male, but transferred to and released mostly by the
females [1]. Aggregation is a simple behavior that has
direct effects on both mature male and mature female
flies, as well as indirect benefits on larvae [2,3].